Journal: Nature Communications

Article Title: Membrane remodeling by FAM92A1 during brain development regulates neuronal morphology, synaptic function, and cognition

doi: 10.1038/s41467-024-50565-w

Figure Lengend Snippet: a Representative electron micrographs showing mitochondrial morphology. Scale bar, 200 nm. b Quantification of mitochondrial diameter, perimeter, and the number of lamellar cristae in the FAM92A1 +/+ ( n = 168), FAM92A1 +/− ( n = 167), and FAM92A1 −/− ( n = 177) groups. Data present mean ± SEM; one-way ANOVA. c OCR of hippocampal mitochondria. d , e Schematic depicting OCR ( d ) and quantified OCR ( e ) in each state. For c and e , data represent mean ± SEM of four biologically independent experiments; two-way ANOVA. f ATP content in the HPC. Data represent mean ± SEM of three biologically independent experiments; one-way ANOVA. g Representative electron micrographs showing myelin sheath, with abnormal myelin indicated by orange arrowheads. Scale bar, 500 nm. h , i Scatter plot with best-fit lines ( h ) and quantification of g -ratio and myelin thickness ( i ). Gaussian curve ( h , inset) showing the reduced axon diameter upon loss of FAM92A1. Data represent mean ± SEM; n = 65 axons per group; Kruskal–Wallis H test ( h ), ANCOVA ( i , g-ratio), and one-way ANOVA ( i , myelin thickness). j Representative electron micrographs showing synaptic contacts, with enlarged SVs indicated by orange arrowheads. Scale bar, 100 nm. k , l Cumulative frequency showing changes in PSD thickness ( k ) and length ( l ). Gaussian curve showing the reduced PSD thickness (inset, k ) and elongated PSD length (inset, l ) upon loss of FAM92A1. For ( a , b ) and ( g – l ) data from three mice per group. m Representative electron micrographs (top) and 3D reconstruction (bottom) of presynaptic compartments. Scale bar, 200 nm. n Schematic illustrating the method for quantifying SVs using FIB-SEM data. o – q Quantification of SV number ( o ), ratio ( p ), and area ( q ). Data represent mean ± SEM; n = 18 sections per group ( o , p ) and data presented as minimum to maximum values (whiskers) and the 25th to 75th percentiles (box), with the mean value within the box; n = 7417 SVs for FAM92A1 +/+ and n = 11,721 SVs for FAM92A1 −/− ( q ); unpaired two-tailed Student’s t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001. Source data and exact p -values are provided as a Source Data file.

Article Snippet: The target volumes in the hippocampus were imaged by a Zeiss FIB-SEM (Crossbeam 550 with Gemini 2 optics, Carl Zeiss Microscopy GmbH, Jena, Germany) using a 2.5 nm pixel size with 5 nm milling depth.

Techniques: Two Tailed Test